Polymorphonuclear Leukocyte Chemotactic Activity
نویسندگان
چکیده
serum and guinea pig serum treated with preformed immune complexes. Normal rabbit sera, guinea pig sera, or rabbit sera deficient in C6 were treated with homologous antibovine serum-albumin-bovine serum-albumin complexes. Sera so treated contained one major PMN chemotactic factor which was heat-stable (56 C for 30 min), had a molecular weight of approximately 15,000, and did not require the presence of C6 for its generation. This factor appears to be analogous to C5a.
منابع مشابه
Polymorphonulcear leukocyte chemotaxis toward oxidized lipid components of cell membranes
Polymorphonuclear leukocyte chemotaxis has been elicited by oxidized arachidonic acid and other oxidized polyenoic lipids in the Boyden micropore filter assay system. This chemotactic activity was observed in the absence of serum and chemotactic proteins. The esterfied arachidonic acid present in plasma membranes may be a precursor of chemotactic messages as well as prostaglandins in vivo.
متن کاملDefective polymorphonuclear leucocyte chemotaxis in rheumatoid arthritis associated with a serum inhibitor.
Cellular and/or serum components of polymorphonuclear leucocyte chemotaxis were assessed in 21 patients with rheumatoid arthritis. No difference in the chemotactic migration of control and patient cells in response to a number of chemotactic solutions could be detected (P = 0.38). Deficient generation of chemotactic activity in patient sera (P = 0.58) as compared to control sera (P = 0.014) aft...
متن کاملBlood polymorphonuclear leukocyte chemotaxis during experimental Escherichia coli bovine mastitis.
The relationship between the severity of experimental Escherichia coli mastitis and the chemotactic response of blood polymorphonuclear leukocytes was investigated before and during mastitis. Experimental E. coli mastitis was induced in 10 healthy cows by inoculation of the rear right quarters with 10(3) cfu of E. coli. Cows were classified into two groups based on the severity of the mastitis....
متن کاملStimulation of human eosinophil and neutrophil polymorphonuclear leukocyte chemotaxis and random migration by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid.
A human platelet lipoxygenase-generated product of arachidonic acid, identified by thin-layer chromatographic and mass spectrometric properties as 12-L-hydroxy-5,8,10,14-eicosatertraenoic acid (HETE), was selectively chemotactic in vitro for human polymorphonuclear leukocytes (PMN), as compared to mononuclear leukocytes, with a preference for eosinophils. Preincubation of PMN with partially-pur...
متن کاملPolymorphonuclear Leukocyte and Monocyte
its elaboration. The chemotactic activity of crudeculture supernate was heat stable (56°C for 30 min), trypsinand pronase-sensitive, and neuraminidase resistant. Characterization of the chemotactic activity by gel filtration (Sephadex G-75) showed two active fractions, one with mol wt >100,000 and the other <10,000. In studies designed to relate these chemotactic factors to collagen, we have co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003